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Amid the strong national and global demand for the imaging of animal tissues, the development of various imaging techniques for direct, non-invasive observation and imaging of various biological tissues and cells in animals has been made in recent years. In particular, optical imaging techniques such as bioluminescence imaging (BLI), fluorescence imaging (FLI), fluorescence molecular tomography (FMT) and fluorescence imaging (FMT) have received particular attention.
As described in Patent Document 1, known examples of the bioluminescence imaging technique are imaging by an imaging system using luciferase that is a protein encoded by firefly luciferase, or imaging by an imaging system using a substrate of luciferase.
Also, as described in Patent Document 2, known examples of the fluorescence imaging technique include a fluorescence-based imaging technique using a substrate that is known to be taken up by a target cell and emits fluorescence upon cleavage. In this technique, the substrate is first allowed to accumulate in the target cell, and then cleavage of the substrate is induced by administration of a cleavage-inducing agent (chemical agent). Subsequently, the accumulation of fluorescence is imaged in the target cell.
The above-described fluorescence imaging technique, however, requires administration of a cleavage-inducing agent and a substrate before the fluorescence is observed. In addition, the fluorescence generated in the target cell gradually disappears due to further degradation of the substrate. Thus, the fluorescence imaging technique is not suitable for the real-time observation of the target cell in vivo.
Thus, as described in Patent Document 3, various studies have been made for development of an imaging technique that uses the autofluorescence of a target cell. For example, Patent Document 4 describes a technique for observing the autofluorescence of cells in vivo by administering a substrate of a substrate-decomposing enzyme to a target cell and observing the autofluorescence of the target cell in the light path of the substrate after the substrate has been decomposed by the substrate-decomposing enzyme. This technique uses, as a substrate, porphyrin, which has a high fluorescence quantum yield. Also, Patent Document 5 describes a technique for the autofluorescence imaging using two or more substrates of different spectral properties (wavelength of the fluorescence). This technique, however, requires two or more administration steps, and does not allow the autofluorescence to be observed non-invasively
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Turn your AutoCAD drawings into apps with no programming effort. Import your drawings to Google Docs, Intuit GoDraw, and Microsoft Visio, and now import them into AutoCAD as an AutoCAD App.
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1. OS: 64-bit Win10 or later
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6. DirectX: Version 11
7. Internet: Internet connection required
8. Control: Keyboard and mouse (SteerPole/JoyPads supported