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a multivariate statistical method was successfully used for detection and assessment of hydrophobicity in ionic liquids. this paper reviews the development of the experimental design methods for estimating the relative hydrophobicity of ils. experimental design methods for such studies are also discussed, and the article concludes with a brief discussion of the future trends that could help to design better ils in terms of hydrophobicity.
phosphorous (p) is one of the major nutrient restrictions on the productivity and distribution of terrestrial plants. without adequate p, gas exchange will be limited by p deficiency of the plant rather than other environmental factors such as temperature, water stress, or nutrient deficiencies. despite the ability of leaves to change p allocation to different cellular components, the general p uptake capacity of plants has rarely been examined under p deficiency. here we measured p content of cellular components of tobacco leaves and examined p uptake of those leaves when exposed to different p supply of medium to evaluate p uptake capacity. the main p transport compounds in tobacco leaves are p-rich protein and p-poor phytate/phytate-containing compounds, which potentially limit p uptake. however, our p contents of cellular components were correlated with p supply of the medium, and p uptake capacity was only significantly limited under low p conditions. we hypothesized that the wide difference in p content among various components was the result of p-responsive elements of p-rich proteins and phytate/phytate-containing compounds, and that the n content of the group mainly responsible for p uptake determined the difference in p uptake capacity. these results provide insight into the improved understanding of p transport and uptake mechanism of plants under different p supply to better inform future multi-nutrient deficiency studies.