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Category:Audio software for LinuxDiabetes is the most common chronic disease in the world and the onset of which is frequently preceded by a pre-diabetic phase. In the early pre-diabetic phase, the patients may progress to the development of early or overt diabetes depending on their response to environmental factors, and due to the loss of β-cell function. The development of type 2 diabetes is also often associated with a decrease in β-cell function and mass. It has previously been shown that a 40% weight loss through a low-calorie diet and physical exercise improves glycemic control in patients with type 2 diabetes [@pone.0048879-Barnard1]–[@pone.0048879-Yamada1]. Our present study clearly shows that the same treatment regime significantly reduces plasma glucose and insulin levels and causes a significant improvement in oral glucose tolerance in mice. With progression of the disease, we observed a decrease in insulin secretion in mice treated with diet and exercise. This decrease in insulin levels in *db/db* mice might be due to a compensatory decrease in insulin levels to keep euglycemia. Diabetic patients and *db/db* mice have similar endocrine pancreas characteristics. These data further support the use of *db/db* mice as an animal model to study pathophysiological mechanisms of diabetes.

Diet and physical activity have been shown to reduce the incidence of diabetes in humans [@pone.0048879-Allison1]–[@pone.0048879-Yamada1]. This study was performed to determine whether a dietary intervention would be able to reverse the metabolic abnormalities seen in early diabetic mice [@pone.0048879-Unger1] that have been shown to respond to a weight loss and exercise intervention. Our present study shows that the dietary intervention improves metabolic parameters such as glycemic control and insulin secretion. We also observed that this treatment reduced the weight of the *db/db* mice in a time-dependent manner. The increased insulin levels in *db/db

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